. Author manuscript; available in PMC: 2012 May 30.

Published in final edited form as: Chem Biol Interact. 2011 Jan 27;191(1-3):147–152. doi: 10.1016/j.cbi.2011.01.023

Table 1.

Effect of Mg²⁺ on the NADH Dissociation and Association Rates with ALDH2.

[Mg²⁺]	k₅	K_D	k₋₅^a
(µM)	(s⁻¹)	(µM)	(µM⁻¹s⁻¹)
0	[8.3]	[468]	[0.018]
20	[4.2]	[317]	[0.013]
60	-	119	-
200	1.4	35	0.040
600	-	16	-
2000	0.5	12	0.04
6000	0.4	12	0.03

k₋₅ = k₅/K_D

[ ] indicates the K_D values significantly exceeded the upper NADH concentration limit reached during NADH titrations. The k₅ values may be underestimated and reflect the rate of mixing rather the rate of NADH dissociation causing the k₋₅ values to also be underestimated.