Table 1. Inflammatory Gene Expression within the Hippocampus of P4 males 2 and 24 hours after E.coli infection.
P4 male pups were treated with either PBS or E.coli infection and brains were collected either 2 or 24 hours after injection (n = 3 samples/treatment group at each time point). RNA was extracted from the hippocampus and quantitative real-time PCR determined that (A) at 2 hours, 18 genes were significantly up-regulated when compared to PBS treated controls; and (B) at 24 hours, 8 genes were significantly up-regulated and 1 gene was significantly down-regulated when compared to PBS treated controls. Genes highlighted in bold are similarly activated by E.coli and LPS (see Table 2). Genes highlighted in italics were affected at 2 (A) and 24 hours (B).
| A. Inflammatory Gene Expression 2 hr after E. coli Treatment | ||||
|---|---|---|---|---|
| Gene Symbol |
Description | Accession No. | Fold-Change from PBS control |
p - value |
| Casp1 | Caspase 1 | NM_012762 | +5.98 | 0.009263 |
| Ccl4 | Chemokine (C-C motif) ligand 4 / MIP-1β | NM_053858 | +1.95 | 0.013928 |
| Ccl7 | Chemokine (C-C motif) ligand 7 / MCP-3 | NM_001007612 | +4.10 | 0.002167 |
| Ccr1 | Chemokine (C-C motif) receptor 1 | NM_020542 | +3.80 | 0.001394 |
| Ccr10 | Chemokine (C-C motif) receptor 10 | XM_343968 | +2.29 | 0.005063 |
| Ccr5 | Chemokine (C-C motif) receptor 5 | NM_053960 | +3.01 | 0.025221 |
| Cx3cl1 | Chemokine (C-X3-C motif) ligand 1 / Fractalkine | NM_134455 | +2.10 | 0.002171 |
| Cxcl1 | Chemokine (C-X-C motif) ligand 1 / GRO-KC | NM_030845 | +2.41 | 0.028021 |
| Cxcl10 | Chemokine (C-X-C motif) ligand 10 | NM_139089 | +2.46 | 0.031596 |
| Cxcl2 | Chemokine (C-X-C motif) ligand 2 | NM_053647 | +3.10 | 0.016091 |
| Cxcl9 | Chemokine (C-X-C motif) ligand 9 | NM_145672 | +2.58 | 0.007728 |
| Il13ra1 | Interleukin 13 receptor, alpha 1 | NM_145789 | +2.40 | 0.000645 |
| Il18 | Interleukin 18 | NM_019165 | +2.81 | 0.004295 |
| Il1r1 | Interleukin 1 receptor, type I | NM_013123 | +2.16 | 0.005082 |
| Il1r2 | Interleukin 1 receptor, type II | NM_053953 | +1.90 | 0.044168 |
| Il6ra | Interleukin 6 receptor, alpha | NM_017020 | +2.78 | 0.000335 |
| Il6st | Interleukin 6 signal transducer | NM_001008725 | +2.18 | 0.003293 |
| Tnfrsf1b | Tumor necrosis factor receptor superfamily, member 1b | NM_130426 | +2.05 | 0.012533 |
| B. Inflammatory Gene Expression 24 hr after E. coli Treatment | ||||
|---|---|---|---|---|
| Gene Symbol |
Description | Accession No. | Fold-Change from PBS control |
p - value |
| Casp1 | Caspase 1 | NM_012762 | +10.78 | 0.044 |
| Ccl12 | Chemokine (C-C motif) ligand 12 / MCP-5 | XM_213425 | +3.33 | 0.03079 |
| Ccl2 | Chemokine (C-C motif) ligand 2 / MCP-1 | NM_031530 | +5.76 | 0.00094 |
| Ccl3 | Chemokine (C-C motif) ligand 3 / MIP-1α | NM_013025 | −2.47 | 0.0343 |
| Cd14 | CD14 molecule | NM_021744 | +2.31 | 0.00429 |
| Il10 | Interleukin 10 | NM_012854 | +8.12 | 0.00016 |
| Il1a | Interleukin 1 alpha | NM_017019 | +3.93 | 0.02459 |
| Il1b | Interleukin 1 beta | NM_031512 | +1.98 | 0.04738 |
| Il1r1 | Interleukin 1 receptor, type I | NM_013123 | +2.73 | 0.01057 |