Table 2. Inflammatory Gene Expression within the Hippocampus of P4 males 2 and 24 hoursafter LPS treatment.
P4 male pups were treated with either PBS or LPS and brains were collected either 2 or 24 hours after injection (n = 3 samples per treatment group at each time point). RNA was extracted from the hippocampus and quantitative real-time PCR determined that (A) at 2 hours, 26 genes were significantly up-regulated and 12 genes were significantly down-regulated when compared to PBS-treated controls; and (B) at 24 hours, 24 genes were significantly up-regulated and 2 genes were significantly down-regulated when compared to PBS treated controls. Genes highlighted in bold are similarly activated by E.coli and LPS (see Table 1). Genes highlighted in italics were affected at 2 (A) and 24 hours (B).
| A. Inflammatory Gene Expression 2 hr after LPS Treatment | ||||
|---|---|---|---|---|
| Gene Symbol |
Description | Accession No. | Fold-Change from PBS control |
p - value |
| Bcl6 | B-cell CLL / lymphoma 6 | XM_221333 | −4.68 | 0.00035 |
| Ccl11 | Chemokine (C-C motif) ligand 11 | NM_019205 | +2.75 | 0.05255 |
| Ccl12 | Chemokine (C-C motif) ligand 12 / MCP-5 | XM_213425 | +3.84 | 0.000902 |
| Ccl17 | Chemokine (C-C motif) ligand 17 / | NM_057151 | −2.04 | 0.005814 |
| Ccl2 | Chemokine (C-C motif) ligand 2 / MCP-1 | NM_031530 | +8.29 | 0.027037 |
| Ccl7 | Chemokine (C-C motif) ligand 7 / MCP-3 | NM_001007612 | +15.85 | 0.011647 |
| Ccr3 | Chemokine (C-C motif) receptor 3 | NM_053958 | +1.95 | 0.040844 |
| Ccr4 | Chemokine (C-C motif) receptor 4 | NM_133532 | +1.90 | 0.015109 |
| Ccr6 | Chemokine (C-C motif) receptor 6 | NM_001013145 | +2.02 | 0.032638 |
| Csf2 | Colony stimulating factor 2 (granulocyte-macrophage) | XM_340799 | +4.04 | 0.019204 |
| Csf3 | Colony stimulating factor 3 (granulocyte) | NM_017104 | +2.77 | 0.011047 |
| Cxcl1 | Chemokine (C-X-C motif) ligand 1 / GRO-KC | NM_030845 | +18.08 | 0.000483 |
| Cxcl10 | Chemokine (C-X-C motif) ligand 10 | NM_139089 | +6.69 | 0.017281 |
| Cxcl11 | Chemokine (C-X-C motif) ligand 11 | NM_182952 | +6.53 | 0.002613 |
| Cxcl12 | Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) | NM_022177 | −3.52 | 0.000271 |
| Cxcl2 | Chemokine (C-X-C motif) ligand 2 | NM_053647 | +20.49 | 0.003258 |
| Cxcl6 | Chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2) | NM_022214 | −2.87 | 0.030606 |
| Fos | FBJ osteosarcoma oncogene / c-fos | NM_022197 | +17.05 | 0.009266 |
| Ifna1 | Interferon-alpha 1 / IFN alpha 1 | NM_001014786 | +2.22 | 0.004677 |
| Ifnb1 | Interferon beta 1, fibroblast / IFN b | NM_019127 | +3.47 | 0.001946 |
| Il10 | Interleukin 10 | NM_012854 | +3.26 | 0.031186 |
| Il10ra | Interleukin 10 receptor, alpha | NM_057193 | −2.00 | 0.038764 |
| Il16 | Interleukin 16 | XM_218851 | −3.34 | 0.003645 |
| Il1b | Interleukin 1 beta | NM_031512 | +3.56 | 0.000657 |
| Il1f6 | Interleukin 1 family, member 6 | XM_231099 | +2.71 | 0.051029 |
| Il1r1 | Interleukin 1 receptor, type I | NM_013123 | −1.90 | 0.014067 |
| Il1r2 | Interleukin 1 receptor, type II | NM_053953 | +2.36 | 0.004793 |
| Il2rg | Interleukin 2 receptor, gamma | NM_080889 | −1.95 | 0.007798 |
| Il3 | Interleukin 3 | NM_031513 | +2.67 | 0.053324 |
| IL6 | Interleukin 6 | NM_012589 | +2.93 | 0.003175 |
| Il6ra | Interleukin 6 receptor, alpha | NM_017020 | +2.07 | 0.002653 |
| Itgb2 | Integrin beta 2 | XM_001069791 | −2.21 | 0.001508 |
| Lta | Lymphotoxin alpha (TNF superfamily, member 1) | NM_080769 | +4.88 | 0.004546 |
| Nfkbia | Nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha | XM_343065 | +2.40 | 0.000263 |
| Tnf | Tumor necrosis factor (TNF superfamily, member 2) | NM_012675 | +4.33 | 0.000179 |
| Tnfrsf1a | Tumor necrosis factor receptor superfamily, member 1a | NM_013091 | −1.83 | 0.033447 |
| Tnfrsf1b | Tumor necrosis factor receptor superfamily, member 1b | NM_130426 | −2.79 | 0.041099 |
| Tollip | Toll interacting protein | XM_341961 | −2.99 | 0.019193 |
| B. Inflammatory Gene Expression 24 hr after LPS Treatment | ||||
|---|---|---|---|---|
| Gene Symbol |
Description | Accession No. | Fold-Change from PBS control |
p - value |
| Casp1 | Caspase 1 | NM_012762 | +11.13 | 0.000366 |
| Ccl2 | Chemokine (C-C motif) ligand 2 / MCP-1 | NM_031530 | +5.14 | 0.002545 |
| Ccl5 | Chemokine (C-C motif) ligand 5 / RANTES | NM_031116 | +3.12 | 0.013004 |
| Ccl7 | Chemokine (C-C motif) ligand 7 | NM_001007612 | +6.06 | 0.008139 |
| Ccl9 | Chemokine (C-C motif) ligand 9 | NM_001012357 | +2.14 | 0.038714 |
| Ccr1 | Chemokine (C-C motif) receptor 1 | NM_020542 | +6.05 | 0.008251 |
| Ccr5 | Chemokine (C-C motif) receptor 5 | NM_053960 | +8.15 | 2.86E-05 |
| Cd14 | CD14 molecule | NM_021744 | +2.24 | 0.006013 |
| Cd180 | CD180 molecule / Ly78 | XM_226731 | +2.45 | 0.017965 |
| Cd80 | Cd80 molecule | NM_012926 | +2.32 | 0.002781 |
| Cd86 | CD86 molecule | NM_020081 | +2.98 | 0.015176 |
| Cx3cl1 | Chemokine (C-X3-C motif) ligand 1 / Fractalkine | NM_134455 | +2.19 | 0.006843 |
| Cxcl11 | Chemokine (C-X-C motif) ligand 11 | NM_182952 | +3.86 | 0.010901 |
| Cxcl6 | Chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2) | NM_022214 | −2.12 | 0.019716 |
| Cxcl9 | Chemokine (C-X-C motif) ligand 9 | NM_145672 | +2.27 | 0.014916 |
| Hmgb1 | High mobility group box 1 | NM_012963 | +2.20 | 2.2E-06 |
| Il16 | Interleukin 16 | XM_218851 | −2.26 | 0.002948 |
| Il18 | Interleukin 18 | NM_019165 | +3.09 | 0.002811 |
| Il1a | Interleukin 1 alpha | NM_017019 | +3.37 | 0.029365 |
| Il1b | Interleukin 1 beta | NM_031512 | +3.42 | 0.030272 |
| Il6ra | Interleukin 6 receptor, alpha | NM_017020 | +4.84 | 2.3E-06 |
| Il8ra | Interleukin 8 receptor, alpha Mitogen activated protein | NM_019310 | +3.97 | 0.015444 |
| Map3k7 | kinase kinase kinase 7 (Tak1) | XM_232855 | +2.14 | 0.005586 |
| Tlr3 | Toll-like receptor 3 | NM_198791 | +2.56 | 0.000266 |
| Tlr5 | Toll-like receptor 5 | XM_223016 | +4.02 | 0.02792 |
| Ube2n | Ubiquitin-conjugating enzyme E2N | NM_053928 | +2.94 | 0.012831 |