Fig. 8. GSK3 does not regulate CHOP expression in MEFs and primary astrocytes.
(A) MEFs were pretreated with or without 20 mM lithium chloride for 1 h followed by treatment with 4 µg/ml tunicamycin for 2 or 4 h. (B) Primary mouse astrocytes were pretreated with or without 20 mM lithium chloride for 1 h followed by treatment with 4 µg/ml tunicamycin for 3 or 6 h. Cell lysates were immunoblotted for CHOP and β-actin.