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. Author manuscript; available in PMC: 2012 May 30.
Published in final edited form as: Chem Biol Interact. 2011 Mar 3;191(1-3):346–350. doi: 10.1016/j.cbi.2011.02.028

Figure 2. RT-PCR and Western blot confirming the knock-down of AKR1B1 by siRNA.

Figure 2

A) Equal amount of total RNA isolated from the thoracic muscle tissue of AKR1B1 knockdown rats was subjected to RT-PCR. B) Equal amount of protein obtained from muscle tissue homogenates was subjected to Western blot analysis using AKR1B1 antibodies. Beta–actin and GAPDH were used as internal standards for RT-PCR and Western blot analysis, respectively. #1, 2, 3 are ARKO rats, Control: Wild-type rats.