Figure 4.
PPT modifies ALDH1L2 specifically at Ser375. A, sequence alignment of ALDH1L1 (cytFDH) and ALDH1L2 (mtFDH) depicting the residues flanking the site of 4-PP attachment (Ser354). Two adjacent serine residues (Ser374 and Ser375) are present at this location in mtFDH. B, SDS-PAGE of recombinant mtFDH mutant constructs after incubation with fluorescent reporter and either recombinant PPT (+PPT) or water (control). Upper panel, SDS-PAGE visualized under UV irradiation. Lower panel, SDS-PAGE after Coomassie staining. NC refers to the S374/375A double mutant.