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. Author manuscript; available in PMC: 2012 May 30.
Published in final edited form as: Chem Biol Interact. 2011 Jan 14;191(1-3):129–136. doi: 10.1016/j.cbi.2011.01.008

Figure 4.

Figure 4

PPT modifies ALDH1L2 specifically at Ser375. A, sequence alignment of ALDH1L1 (cytFDH) and ALDH1L2 (mtFDH) depicting the residues flanking the site of 4-PP attachment (Ser354). Two adjacent serine residues (Ser374 and Ser375) are present at this location in mtFDH. B, SDS-PAGE of recombinant mtFDH mutant constructs after incubation with fluorescent reporter and either recombinant PPT (+PPT) or water (control). Upper panel, SDS-PAGE visualized under UV irradiation. Lower panel, SDS-PAGE after Coomassie staining. NC refers to the S374/375A double mutant.

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