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. Author manuscript; available in PMC: 2012 May 17.
Published in final edited form as: Langmuir. 2011 Apr 19;27(10):6107–6115. doi: 10.1021/la104924c

Figure 1.

Figure 1

(A) DOPC vesicles labelled with NBD-PE (green) coexisting with 4:1 DOPC:CL vesicles (unlabelled) and Alexa Fluor 568 labelled cyt c (red). The cyt c only binds detectably to the CL-containing vesicles. (B) Phase separated vesicles containing 10% CL, 25% chol, 27.5% DOPC, 37.5% DPPC (0.5% Rh-PE, 0.5% NBD-PE). The Rh-PE (red) labels the Ld phase and the NBD-PE (green) labels the Lo phase. (C) Phase separated CL-containing vesicles labelled only with the NBD-PE lipid probe (green) in solution with Alexa Fluor 568 labelled cyt c (red). The cyt c only binds detectably to the CL-containing Ld domains. The insets represent the separate red and green fluorescence channels that are collected and superimposed to create the composite image.