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. 2011 Mar 2;4(3):158–163. doi: 10.1093/ndtplus/sfr010

Fig. 2.

Fig. 2.

(A) Wild-type AVPR2-GFP transiently expressed in MDCK cells and stained with the Golgi marker G58K. Wild-type AVPR2-GFP localizes to cell–cell contacts of MDCK cells and to the Golgi region. (B). AVPR2-L170P-GFP transiently expressed in MDCK cells and stained with the Golgi marker G58K. AVPR2-L170P-GFP does not seem to localize to the Golgi compartment. (C) AVPR2-L170P-GFP transiently expressed in MDCK cells and stained with the ER marker calreticulin. AVPR2-L170P-GFP localizes to intracellular structures with some overlap with the ER marker calreticulin. (D) AVPR2-L170P-GFP transiently expressed in MDCK cells. Cells were loaded with Lysotracker Red before fixation, which shows that cells expressing AVPR2-L170P-GFP had larger lysosomes than surrounding cells, although AVPR2-L170P-GFP did not colocalize, which could be due to degradation of the GFP-tag in lysosomes. Please note that samples B, C and D have been stained with antibody against the GFP tag since they were otherwise not bright enough for imaging. Scale bar is 10 μm.