Figure 5.

SS-31 improves kidney mitochondrial function after IR injury. Rats were subcutaneously treated with saline or SS-31 (2 mg/kg) 30 minutes before occlusion of renal blood flow for 45 minutes. Treatment was repeated just before onset of reperfusion. Mitochondria were freshly isolated from kidney tissue obtained at 5, 20, or 60 minutes after reperfusion. ATP was determined from whole kidney tissue obtained at the same time points. (A) Mitochondrial respiration was measured by oxygen consumption using isolated mitochondria with complex I substrates (glutamate/malate) and ADP was added to initiate state 3 respiration. (B) Whole kidney tissue ATP content was determined using the luciferase assay. n = 4 to 8 in each group. **P < 0.01 and ***P < 0.001 compared with Sham; #P < 0.05 comparing saline and SS-31 treatment.