Figure 6.

SS-31 minimizes renal tubular cell detachment at 1 hour after IR injury. Rats were subcutaneously treated with saline or SS-31 (2 mg/kg) 30 minutes before occlusion of renal blood flow for 45 minutes. Treatment was repeated just before onset of reperfusion, and kidneys were sectioned and stained for β1-integrin by immunohistochemisty and for cell detachment using PAS stain. Panels a through c show β1-integrin staining (reddish-brown stain as shown by arrow) in (A) Sham, (B) saline-treated IR, and (C) SS-31-treated IR rats. β1-integrin was clearly localized to the basal membrane of the tubular cell in Sham and SS-31-treated IR animals, whereas it was diffused throughout the cell in saline-treated IR animals. Panels D through F show that tubular cells are attached to the basement membrane in (D) Sham and (F) SS-31-treated IR animals, but detached cells, some viable (shown by arrow), were clearly found within the tubular lumen of (E) saline-treated IR animals. Magnification: ×600.