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Af17 regulates mRNA and protein expression of ENaC. (A) Real-time RT-qPCR for expression of ENaC genes in kidney of mice fed the normal Na+ diet, with β-actin as internal control. n = 3 mice/group. (B) Western blots for expression of proteins as indicated, with β-actin as internal control. For αENaC, the approximately 30-kD band was excluded in data analyses. me2K79 and me2K9: histone H3 dimethylated K79 and K9, respectively. n = 4 mice/group. In all cases, *P < 0.05 versus Af17+/+.
