Figure 7.

Co-expression of dominant negative caveolin-1 (CAVS80E) or downregulation of CAV-1 prevents ROMK channel activity declining. Typical K currents measured with the perforated whole cell recording in HEK293 cells co-transfected with GFP-ROMK1 + siRNA of caveolin-1 (siRNACAV) (A) or ROMK1 + CAVS80E (B) 24 hours after transfection. The same construct as described in Figure 5B was used to downregulate caveolin-1. The HEK cells were treated with BFA, and K currents were measured from −100 to 60 mV with a 20-mV increment. (C) A line graph summarizes the above experiments (each point represents at least four experiments at −100 mV) showing the effects of siRNA and CAVS80E on K currents. *Significant difference from the corresponding control (0 hours).