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. Author manuscript; available in PMC: 2011 Nov 24.
Published in final edited form as: Chem Biol. 2010 Nov 24;17(11):1212–1222. doi: 10.1016/j.chembiol.2010.09.012

Figure 7. Selected MS/MS spectra of the modified universal stress protein G peptides (aa 70–83).

Figure 7

AOA/ANL-metabolically-modified proteins were clicked to tag 1, 4 or 6 via CuAAC and then processed via in-solution proteomic strategy. Selected MS/MS spectra were derived from the results of MS analysis of those streptavidin-enriched and Na2S2O4-eluted modified peptides. (A) MS/MS spectrum of the modified peptide LQTM*VGHFSIDPSR (M* = AOA + tag 1/CuAAC/Na2S2O4-cleavage adduct, the molecular weight of M* = 315). (B) MS/MS spectrum of the modified peptide LQTM*VGHFSIDPSR (M* = ANL + tag 4/CuAAC/Na2S2O4-cleavage adduct, the molecular weight of M* = 301). (C) MS/MS spectrum of the modified peptide LQTM*VGHFSIDPSR (M* = AOA + tag 6/CuAAC/Na2S2O4-cleavage adduct, the molecular weight of M* = 393). See also Tables S2–S4.