Figure 3.

Free fatty acid induced lipid accumulation in L-02 cells. A: L-02 cells were incubated with a free fatty acid (FFA) mixture (oleate and palmitate at the ratio of 2:1) for 24 h. Intracellular lipid accumulation was evaluated after Nile red staining. Results were expressed as mean ± SE of three independent experiments. ^bP < 0.01 vs control group; B: Representative micrographs showing intracellular lipid accumulation in L-02 cells as observed by phase-contrast microscopy (panels a-c) and fluorescence microscopy (panels d-f). Panels a/d, b/e and c/f are control cells, cells treated with 0.5 and 1 mmol/L FFA, respectively; C: Triglyceride levels in L-02 cells treated with 1 mmol/L FFA. Results were expressed as mean ± SE of three independent experiments. ^bP < 0.01 vs control group.