Table 6.

Western blot densitometry data (arbitrary units)

Protein	STC	STC + S	FAT	FAT + S	FRU	FRU + S	SUC	SUC + S
Phospho-AMPK	4.34 ± 0.36	5.13 ± 0.31	4.17 ± 0.20	4.57 ± 0.30	4.43 ± 0.19	4.41 ± 0.17	4.44 ± 0.17	4.44 ± 0.12
Total AMPK	14.5 ± 0.63	18.2 ± 1.00*,‡	12.9 ± 0.98†	14.5 ± 0.7	14.0 ± 1.4†	17.4 ± 0.9*	17.6 ± 0.83	18.1 ± 0.9
Phospho-AMPK/total AMPK	0.300 ± 0.025	0.281 ± 0.008	0.330 ± 0.016†	0.315 ± 0.011	0.334 ± 0.035†	0.257 ± 0.014*	0.254 ± 0.012	0.246 ± 0.012
Phospho-Akt	1.49 ± 0.02	1.52 ± 0.05	1.45 ± 0.02†	1.46 ± 0.02	1.51 ± 0.03	1.45 ± 0.03§	1.57 ± 0.04	1.58 ± 0.04
Total Akt	8.50 ± 0.52	10.05 ± 0.74	7.45 ± 0.54†	8.73 ± 0.86	7.96 ± 0.72	9.25 ± 0.50	9.95 ± 0.50	10.99 ± 1.19
Phospho-Akt/total Akt	0.180 ± 0.011	0.154 ± 0.008	0.200 ± 0.012	0.176 ± 0.018	0.197 ± 0.016	0.160 ± 0.009*	0.160 ± 0.007	0.148 ± 0.011
Phospho-FoxO3a	6.00 ± 0.21	6.62 ± 0.22	6.06 ± 0.23	6.16 ± 0.33	6.34 ± 0.22	5.93 ± 0.25	6.10 ± 0.21	5.77 ± 0.10
Total FoxO3a	4.68 ± 0.04	4.83 ± 0.07	4.63 ± 0.06	4.71 ± 0.10	4.75 ± 0.07	4.65 ± 0.06	4.69 ± 0.06	4.61 ± 0.05
Phospho-FoxO3a/total FoxO3a	1.28 ± 0.04	1.37 ± 0.03	1.31 ± 0.04	1.31 ± 0.05	1.33 ± 0.03	1.27 ± 0.04	1.300 ± 0.04	1.25 ± 0.02

Values are means ± SEM. AMPK, AMP activated protein kinase.

^*P <0.05 vs. same diet with low salt.

^†P <0.05 vs. SUC.

^‡P <0.05 vs. FAT + S.

^§P <0.05 vs. SUC + S.