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Day and night variation in reduced glutathione (GSH) content in red blood cells after in vitro treatment with melatonin. The points (□) and (■) represent the effect of melatonin in diurnal and nocturnal blood samples respectively. Dose-dependent variation was observed in both diurnal and nocturnal samples in response to in vitro treatment. Significant change was observed at 10−9 M concentration of melatonin (*P < .05). GSH content is expressed as milligram per millilitre packed RBCs.
