Western blotting for determination of increase in Bax:Bcl-2 ratio leading to mitochondrial release of cytochrome c into the cytosol in glioblastoma A172, LN18, and SNB19 cells. Treatments: control, 0.5 μM 4-HPR (5 days), 200 units/ml IFN-γ (2 dads), and 0.5 μM 4-HPR (3 days) + 200 units/ml IFN-γ (2 days). Representative Western blots (n ≥ 3) and bar graphs show changes in: (A) Bax:Bcl-2 ratio, (B) 15 KD cytochrome c in mitochondria, and (C) 15 kD cytochrome c in cytosol.