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Activation of molecular components of the extrinsic and intrinsic pathways of apoptosis. Treatments: control, 0.5 μM 4-HPR for 72 h, 25 μM GST for 24 h, and 0.5 μM 4-HPR for 48 h (pretreatment) + 25 μM GST for 24 h. Representative Western blots to show activation and level of molecules involved in extrinsic and intrinsic pathways of apoptosis. Expression of β-actin was used as a loading control. All experiments were conducted in triplicates.
