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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Cell Tissue Res. 2011 May 12;344(3):567–576. doi: 10.1007/s00441-011-1171-0

Figure 1.

Figure 1

qRT-PCR and RT-PCR analysis of steady-state Tnfrsf9 mRNA levels and presence of alternatively spliced forms of Tnfrsf9 in the mouse uterus during implantation and decidualization . (a) qRT-PCR analysis of relative mRNA levels in nonimplantation site (NIS) compared to implantation site (IS) tissue segments on Day 3.5-8.5 of pregnancy. Bars in the graphs bearing similar letters are not significantly different and represent mean ± SEM (N=4). (b) qRT-PCR analysis of relative mRNA levels (mean ± SEM, N=4) in bead-induced deciduoma (BID) compared to IS tissue segments on Days 3.5 to 8.5 of pseudopregnancy and pregnancy, respectively. (c) RT-PCR analysis of Tnfrsf9 splice variant expression in non-implantation site (NIS) compared to implantation site (IS) tissue segments on Day 5.5-8.5 of pregnancy and Day 7.5 BID deciduoma tissue. RT-PCR results are representative of several (N=3) independent samples. Abbreviations: DOP, day of pregnancy; bp, base pair; D, deciduoma; and – RT, no-reverse transcriptase control.