Fig. 5.

Cell cycle regulation of myosin II activity. (A) Western blot probing two different M-phase and I-phase extract pairs with an antibody against phosphorylated myosin light chain (MLC-P). For a loading control, the blot was also probed with a monoclonal antibody against actin (Act). (B) Addition of phalloidin induces gelation-contraction in I-phase extracts. Phalloidin was added and drops were immediately transferred to room temperature for imaging. The 60-minute time point is shown for each condition. Scale bar: 500 μm.