Figure 3.

Real-time monitoring of linker histone H1 induced DNA compaction in a hydrodynamic focusing device. (a) Simulation results (top) and birefringence data (bottom) close to the confluence region are contrasted (u_DNA=600 μm s⁻¹). The product of the assembly reaction appears in the diffusion cone of side and main stream components due to its highly increased viscosity. (b) Simulated H1 concentration profile of the whole device (x=−200–12000 μm). Dependence of the N∕P ratio on the position along x (c) and the time t (d) in the middle of the outlet channel (y=0).