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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Exp Gerontol. 2011 Mar 3;46(7):542–548. doi: 10.1016/j.exger.2011.02.011

Figure 5. Effect of enalapril and losartan on phosphorylation of p65 in the aged rat.

Figure 5

Cytosolic and nuclear proteins were prepared to determine the phosphorylation levels of p65. Ser 536 phosphorylation of p65 and IKKαβ phosphorylation were detected by Western blotting using anti p-p65- (Ser 536) and anti p-IKKαβ-specific polyclonal antibodies (A). Ser 276 phosphorylation of p65 and MSK-1 phosphorylation were detected by Western blotting using anti p-p65- (Ser 276) and anti p-MSK-1-specific polyclonal antibodies (B). Levels were normalized to β-actin in the cytosolic fraction and histone H1 in the nuclear fraction. One representative blot is shown from 3 experiments that yielded similar results. **P<0.01, ***P<0.001 vs. normal aged rat. IKKαβ, IκB kinaseαβ; MSK-1. mitogen- and stress-activated protein kinase-1; ENA, enalapril; LOS, losartan.