Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Exp Gerontol. 2011 Mar 3;46(7):542–548. doi: 10.1016/j.exger.2011.02.011

Figure 6. Effect of enalapril and losartan on NF-κB-dependent pro-inflammatory genes and adhesion molecules expression in the aged rats.

Figure 6

Cytosolic proteins were prepared to determine the expression levels of NF-κB-dependent pro-inflammatory gene and adhesion molecules. Western blotting was performed to detect renal COX-2, iNOS, and 5-LOX levels in cytoplasmic extracts from aged rats (A). NF-κB-dependent Adhesion molecules levels were detected by Western blot using anti VCAM-1 and anti ICAM-1-specific polyclonal antibodies. Levels were normalized to β-actin. One representative blot is shown from 3 experiments that yielded similar results. **P<0.01, ***P<0.001 vs. normal aged rat by ANOVA. COX-2; cyclooxygenase 2, 5-LOX; 5-lipoxygenase, iNOS; inducible NO synthase; VCAM-1; vascular cell adhesion molecule 1, ICAM-1; Inter-Cellular Adhesion Molecule 1, ENA, enalapril; LOS, losartan