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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Insect Physiol. 2011 Feb 17;57(5):620–627. doi: 10.1016/j.jinsphys.2011.02.003

Figure 3.

Figure 3

Northern blot analysis of cpo mRNA. A: Developmental expression pattern of cpo transcripts from day 1 of the first larval instar (L1), day 1 of the second larval instar (L2), day 1 of the third larval instar (L3), day 1 of the fourth larval instar (L4), day 1 pupae (P1), day 1 adult females (A1), and day 7 adult females (A7). Mosquitoes were reared under nondiapause condition at 25°C with a photoperiod of 16 L: 8 D. 5 μg total RNA from each sample was loaded and 18 S rRNA was employed as a loading control. B: Comparison of cpo mRNA in 7 day-old diapausing (D) and nondiapausing (ND) adult females. 1.5 μg mRNA from each sample was loaded and sizes of RNA molecular markers are shown at left.