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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Insect Physiol. 2011 Feb 17;57(5):620–627. doi: 10.1016/j.jinsphys.2011.02.003

Figure 5.

Figure 5

Expression pattern of cpo mRNA by qPCR in short-day (diapause-destined, D) and long-day (nondiapause-destined, ND) mosquitoes in day 1 fourth instar larvae (DL4 and NDL4), day 2 pupae (DP2 and NDP2), day 2 (DA2 and NDA2), 7 (DA7 and NDA7), 14 (DA14 and NDA14), 28 (DA28 and NDA28), and 60 (DA60 and NDA60) adult females. Day 60 diapausing females were transferred to 25°C and long daylength (16L: 8D) for 7 days to break diapause, and nondiapausing females were also transferred as controls (D18–25 and ND18–25). Error bars represent standard deviations of three replicates; 15 individuals were sampled in each replicate. Single asterisk indicates significant difference, with double asterisks and triple asterisks indicating very significant differences between the two tested groups, respectively (*P<0.05, **P<0.01, ***P<0.001, student's t-test).