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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Insect Physiol. 2011 Feb 17;57(5):620–627. doi: 10.1016/j.jinsphys.2011.02.003

Figure 6.

Figure 6

JHIII application terminated C. pipens diapause as indicated by an increase in follicle length and decreased expression level of cpo mRNA evaluated by qPCR. Day 14 diapausing adult females were treated with JHIII at three doses: 5 ng (JH5), 50 ng (JH50), and 500 ng (JH500). Day 14 diapausing adult females (D), nondiapausing adult females (ND), and acetone-treated day 14 diapausing adult females (Acetone), were employed as controls. Expression levels of cpo and primary ovarian follicle lengths were examined 7 days after treatment. Primary follicle lengths are shown as blocked diamonds and expression levels of cpo as columns. Error bars represent standard deviations of three replicates; 15 individuals were sampled in each replicate. Single asterisk and double asterisks indicate significant difference and very significant difference, respectively (*P<0.05, **P<0.01, student's t-test).