. Author manuscript; available in PMC: 2012 May 1.

Published in final edited form as: J Insect Physiol. 2011 Feb 17;57(5):620–627. doi: 10.1016/j.jinsphys.2011.02.003

Table 1.

Primers used for cloning, probe production, and qPCR.

Primer sequence	Purpose	Amplicon size	Efficiency for qPCR(%)
CPP2: (forward) 5 '-GAAGGTTCACTGT/CTGAAA/GGT-3'	For cDNA internal fragment	174 bp	---
CPP3: (reverse) 5'-GTTACTT/CTTGGCA/GAA TTCC-3'	For cDNA internal fragment	174 bp	---
CPP4: (reverse) 5'-CGTACACCTTGCTGTAGATCCT-3'	For 5'-RACE	---	---
CPP5: (forward) 5'-TTCGTCACGTTCAGCACACGGT-3'	For 3'-RACE	---	---
CPP8: (forward) 5'-TCAGTACACAGTCCATCAACGG-3'	For hybridization probe	567 bp	---
CPP9: (reverse) 5'-TACCTAGGTGTCCAGCAGTAAG-3'	For hybridization probe	567 bp	---
^*qRPL19P1 (forward) 5'-GCTTTGTTTGATCGTGTGTGA-3'	For rpl19 qPCR	105 bp	101.4
^*qRPL19P2 (reverse) 5'-AACATATCTCCCCCAATCCAG-3'	For rpl19 qPCR	105 bp	101.4
^*q28SP1 (forward) 5'-ACGTGAAACTGCCTAGGGCTC-3'	For 28SrRNA qPCR	200 bp	97.8
^*q28SP2 (reverse) 5'-TGGGACAAGCAACCAGATG-3'	For 28SrRNA qPCR	200 bp	97.8
^*qCPOP1 (forward) 5'-TTCGTCACGTTCAGCACACGGT-3'	For cpo qPCR	119bp	102.4
^*qCPOP2 (reverse) 5'-GTGTTACTCTTGGCGAATTCC-3'	For cpo qPCR	119bp	102.4
qRPL40P1 (forward) 5'-ACTGCCGCAAGAAGAAGTGT-3'	For rpl40 qPCR	104 bp	98.2
qRPL40P2 (reverse) 5'-GGAATCAATCCAGAAGCACAA-3'	For rpl40 qPCR	104 bp	98.2

indicates that the primer sequence was previously reported in Zhang and Denlinger (2011).