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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Nat Methods. 2011 May 1;8(6):493–500. doi: 10.1038/nmeth.1602

Figure 5.

Figure 5

Comparison of computational alignment of separate brains with co-expression within the same brain. For all images, grey shows N-cadherin (N-Cad) labeling, which serves as the reference signal for alignment to the nc82-labeled target. Magenta, FasII antibody staining; green, GAL4 expression pattern (anti-GFP stain). (a) Wild-type w1118 adult brain. (b–d) Expression patterns of the indicated lines shown as maximum intensity projections of 20X confocal image stacks. (e, g, i) Cross-sectional views of computational alignments of FasII expression from (a) with GAL4 patterns from (b–d). (f, h, j) Matched cross-sectional views of brains expressing the GAL4 lines and labeled with both anti-GFP and anti-FasII to show biological co-localization. OK107 and 201Y expression patterns overlap with FasII (yellow arrows), but C232 expresses in adjacent but non-overlapping brain regions (red arrow). Scale bars, 100 µm.