Fig. 1.
Group I mGluR increases DREAM protein expression. (A, C, and D~F) HEK293 cells were co-transfected with 500 ng of each plasmid expressing DREAM or hippocalcin and 50 ng of pRK5 empty vector, pRK5-mGluR subtypes, or pRK5-mGluR5 F767S (Gαq binding site mutant of mGluR5). Cell lysates were immunoblotted with rabbit anti-DREAM or anti-hippocalcin antibody. (B) pRK5 or pRK5-mGluR5 (200 ng) was transfected into SK-N-MC cells for 36 h before cell lysis. Immunoblotting using mouse anti-DREAM antibody was performed. (E) Cells were treated with MPEP for 15 h before lysis. Lysis was performed by boiling with denaturing lysis buffer after 36 h of cultivation. WT, wild-type.