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. 2011 Mar 17;20(6):986–995. doi: 10.1002/pro.622

Figure 1.

Figure 1

RNAP α-subunit variants. (A) Schematic diagram illustrating the WT α and α-X234–241H variants. WT α interdomain linker, black rectangle; the PreScission protease site substituted in the α-X234–241H interdomain linker, red rectangle; (His)10 tag, blue rectangle. The numbers beneath indicate the amino acid position. (B) Amino acid sequence alignment of the WT α and α-X234–241H interdomain linker. The numbers at the beginning and end of each line indicate the amino acid position. The PreScission protease recognition site in α-X234–241H is shown in emboldened red text, and the cleavage site is indicated with a red arrow.