Figure 1.

Characterization of exosomes from CIITA-transducted tumor cells. (A). MHC class II expression on CIITA-transfected B16F1 tumor cells. The clone cell were analysed by flow cytometry to evaluation expression of MHC class II molecules. (B) exosomes were observed by electron microscopy (Bar=100 nm) (C) cellular proteins and exosomes from mock (B16) or CIITA transduced (CIITA) cells were analyzed by immunoblot analysis with each indicated antibodies against MHC class II, TRP-2, Tsg101, Hsc70, tubulin, and Calnexin. (D) Bead-exosomes complex were incubated with PE-conjugated anti-MHC class I and II antibodies, and analyzed by flow cytometry.