Figure 1.

PAUF enhances the adhesiveness of pancreatic cells in vitro. (A, B) The effects of PAUF overexpression (A) and knockdown (B) on cell adhesion were determined with the adhesion assay using fibronectin, collagen or vitronectin-coated plates. Cells were seeded in 96-well plates coated with each ECM molecule. After 20 min incubation at 37℃, non-adherent cells were washed with PBS and then adherent cells were counted. (C, D) Adhesion activities of PAUF-overexpression (C) or knockdown (D) cells were measured in the presence of anti-PAUF (30 µg/ml), control antibody (heat-inactivated anti-PAUF, 30 µl/ml), or PBS. Data represent mean values ± SE. ^*, P < 0.05, ^**, P < 0.01.