Fig. 10. Procysteine Preserves Phagocytosis of P. aeruginosa in Hyperoxia.
RAW 264.7 cells were exposed to 95% O2 in the presence (Pre: added 24 h prior to; Post, added 24 h after the start of hyperoxic exposure) of 1 mM procysteine. After hyperoxic exposure, RAW 264.7 cells were incubated with either non-mucoid PAO1 (A) or mucoid CF1 (B) for 1 h, and then assayed for Colony Forming Unit. The numbers of colonies were normalized to the macrophage number and expressed as the percentage of the number (which is designated as 100%) prior to the exposures (either hyperoxia or room air). *, p<0.05, compared to the hyperoxic control without procysteine treatments.