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. Author manuscript; available in PMC: 2011 May 31.
Published in final edited form as: Free Radic Biol Med. 2007 Jan 23;42(9):1338–1349. doi: 10.1016/j.freeradbiomed.2007.01.031

Fig. 9. SOD Preserves P. aeruginosa Uptake by Macrophages in Hyperoxia.

Fig. 9

RAW 264.7 cells were exposed to 95% O2 for 24 h in the presence or absence of 250 U/ml superoxide dismutase-1. After hyperoxic exposure, RAW 264.7 cells were incubated with PAO1 for 1 h and stained with phalloidin to visualize the organization of the actin cytoskeleton. PAO1 was pseudocolored as white. At least 50 macrophages per slide were counted and expressed as the number of bacteria/macrophage. Macrophages treated with superoxide dismutase-1, and remained at room air were used as controls. *, p<0.05, compared to the hyperoxic sample without addition of SOD1. Bar, 10 μm.