Figure 5.

IKKα and IKKβ increase tumorigenesis and invasiveness of MCF7 cells. (A) MTT assay was used to analyze the growth rate of indicated cells. Each data represents mean ± SD calculated from three independent experiments, and there are four wells for each point in a single experiment. (B) Assay of colony-forming ability in soft-agar. Each experiment have three 60 mm dishes and each data represents mean ± SD calculated from two independent experiments. (C) Assay of invasive ability of indicated cells. Each experiment have three trans-wells and each data represents mean ± SD calculated from two independent experiments. (D) Western blot analysis of the expression of cyclin D1 and twist protein in the indicated cells.