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. 2011 May 2;12(1):60. doi: 10.1186/1465-9921-12-60

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Copyright ©2011 Dahal et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Prevalence of pulmonary MCs in IPAH patients. Lung tissues from donors and IPAH patients were stained with toluidine blue (TB). The arrow indicates the positive signal (purple/violet stain) for the TB-stained MCs. (A) Representative photomicrographs of lung sections from donor (a) and patients (b) are shown. (B) Total and (C) perivascular MC count of different vessel size are given. (D) Perivascular MCs were further analyzed to identify granulated (a) and degranulated (b) MCs, and an index of granulation (IOG) was determined. (E) IOG (in %) is shown. Each bar represents Mean ± SEM (n = 10-15). *p < 0.05 versus donor/corresponding vessels of donor. Scale = 20 μm.