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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Prostate. 2010 Dec 1;70(16):1799–1808. doi: 10.1002/pros.21216

Table 1.

Human
Primers
Sequence Annealing
Temp. (°C)
ANGPT1 Forward: 5’ ATGGACTGGGAAGGGAACCGAGC 3’
Reverse: 5’ GGGGCCACAAGCATCAAACCACC 3’
69
ANGPT2 Forward: 5’ TAAGCAGCATCAGCCAACCAGGAAA 3’
Reverse: 5’ TTTGTGTTCTGCCTCTGTGGATAGTA 3’
69
VEGFa Forward: 5’ CCTGTGTGCCCCTGATGCGATG 3’
Reverse: 5’ GCTTTCGTTTTTGCCCCTTTCCCT 3’
69
HIF-1α Forward: 5’ GCACAGAAGCAAAGAACCCATTTTC 3’
Reverse: 5’ GGCAGTGGTAGTGGTGGCATTAG 3’
69
RPL13a Forward: 5’ CCTGGAGGAGAAGAGGAAAGAG 3’
Reverse: 5’ TTGAGGACCTCTGTGTATTTGTCAA 3’
60
Mouse Primers
Angpt1 Forward: 5’ GATGGACTGGGAAGGGAACCGAG 3’
Reverse: 5’ CGAACCACCAACCTCCTGTTAGCA 3’
69
Angpt2 Forward: 5’ TTAGCACAAAGGATTCGGACAATGAC 3’
Reverse: 5’ TCTGGTAGTGTAGGCAGGCATTTAG 3’
69
Vegfa Forward: 5’ GTGAGCCTTGTTCAGAGCGGAGA 3’
Reverse: 5’ CGGTGACGATGATGGCGTGGTG 3’
69
Hif-1α Forward: 5’ TTACCTTCATCGGAAACTCCAAAGC 3’
Reverse: 5’ ACTGGGACTGTTAGGCTGGGAAAA 3’
69
Rpl13a Forward: 5’ AGTTTGCTTACCTGGGGCGTCTG 3’
Reverse: 5’ TCCACATTCTTTTCTGCCTGTTTCCG 3’
69