Table 1.
Human Primers |
Sequence | Annealing Temp. (°C) |
---|---|---|
ANGPT1 | Forward: 5’ ATGGACTGGGAAGGGAACCGAGC 3’ Reverse: 5’ GGGGCCACAAGCATCAAACCACC 3’ |
69 |
ANGPT2 | Forward: 5’ TAAGCAGCATCAGCCAACCAGGAAA 3’ Reverse: 5’ TTTGTGTTCTGCCTCTGTGGATAGTA 3’ |
69 |
VEGFa | Forward: 5’ CCTGTGTGCCCCTGATGCGATG 3’ Reverse: 5’ GCTTTCGTTTTTGCCCCTTTCCCT 3’ |
69 |
HIF-1α | Forward: 5’ GCACAGAAGCAAAGAACCCATTTTC 3’ Reverse: 5’ GGCAGTGGTAGTGGTGGCATTAG 3’ |
69 |
RPL13a | Forward: 5’ CCTGGAGGAGAAGAGGAAAGAG 3’ Reverse: 5’ TTGAGGACCTCTGTGTATTTGTCAA 3’ |
60 |
Mouse Primers | ||
Angpt1 | Forward: 5’ GATGGACTGGGAAGGGAACCGAG 3’ Reverse: 5’ CGAACCACCAACCTCCTGTTAGCA 3’ |
69 |
Angpt2 | Forward: 5’ TTAGCACAAAGGATTCGGACAATGAC 3’ Reverse: 5’ TCTGGTAGTGTAGGCAGGCATTTAG 3’ |
69 |
Vegfa | Forward: 5’ GTGAGCCTTGTTCAGAGCGGAGA 3’ Reverse: 5’ CGGTGACGATGATGGCGTGGTG 3’ |
69 |
Hif-1α | Forward: 5’ TTACCTTCATCGGAAACTCCAAAGC 3’ Reverse: 5’ ACTGGGACTGTTAGGCTGGGAAAA 3’ |
69 |
Rpl13a | Forward: 5’ AGTTTGCTTACCTGGGGCGTCTG 3’ Reverse: 5’ TCCACATTCTTTTCTGCCTGTTTCCG 3’ |
69 |