Fig. 3.

Sequencing of cDNA reveals that increased mRNA transcript levels originate from the normal allele. Brain mRNA from GRN mutants was reverse-transcribed, and the resulting cDNA was sequenced. Row A depicts genomic DNA for each GRN mutant; mutant peaks are indicated by colored arrows. Row B depicts cDNA for each GRN mutant, and Row C depicts normal cDNA for comparison. For all GRN mutants tested, mRNA primarily represents the normal allele indicating that the increased transcript levels result from the normal allele