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. 2011 May 10;2(3):e00051-11. doi: 10.1128/mBio.00051-11

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Copyright © 2011 Binet et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 2 — Kinetics of SAM transport in E. coli. (A) Time course of [¹⁴C]SAM uptake in E. coli at 37°C as a function of time. The time course assay was carried out in transport buffer supplemented with 10 µM [¹⁴C]SAM. Time point assays were done in triplicate. (B) Effect of substrate concentration on [¹⁴C]SAM uptake at 37°C. The data represent six independent experiments done in triplicate. The background transport seen with MG1655 (○) was subtracted from the transport seen with ATM915 (MG1655 expressing ctl843 [Δ]) with GraphPad Prism software, and then nonlinear regression analysis was performed to obtain the K_m and V_max values.