. 2011 May 10;2(3):e00051-11. doi: 10.1128/mBio.00051-11

TABLE 1 .

Bacterial strains and plasmids

Strain or plasmid	Genotype/description	Reference or source
E. coli strains
DH5α	F⁻ φ80 Δ(lacZY-argF)U169 deoR recA1 endA1 phoA hsdR17 supE44 λ⁻ thi-1 gyrA96 relA1 Δ(lacZ)M15	54
MG1655	F⁻ λ⁻ ilvG rfb-50 rph-1	E. coli Genetic Stock Collection
MC4100	F⁻ araD139 Δ(argF-lac)U169 rpsL150 relA1 deoC1 rbsR fthD5301 fruA25 λ⁻	55
BW25113	Δ(araD-araB)567 ∆lacZ4787 (::rrnB-3) λ⁻ rph-1 Δ(rhaD- haB)568 hsdR514	56
ATM609	BW25113 transformed with pKD46	56
ATM770	∆metK::kan/pREF71	Allelic exchange mutant of ATM609/pREF71
ATM777	MC4100/pREF73	Cm^r transformant of MC4100 with pREF73
ATM778	∆metK::kan/pREF73	Km^r transductant of ATM777 with P1 grown on ATM770
NC13	MC4100 ∆pfs(8-226)::kan	17
ATM1113	∆metK::kan/pREF73/pREF77	Ap^r transformant of ATM778 with pREF77
ATM1114	∆metK::kan/pREF73/pRAK368	Ap^r transformant of ATM778 with pRAK368
ATM1115	MG1655 ∆pfs::kan	Km^r transductant of MG1655 with P1 grown on NC13
ATM915	MG1655/pRAK368	Ap^r transformant of MG1655 with pRAK368
ATM1116	∆pfs::kan/pRAK368	Km^r transductant of ATM915 with P1 grown on NC13
ATM1117	∆metK::kan/pREF73/pUC18	Ap^r transformant of ATM778 with pUC
Plasmids
pKD46	P_ara gam bet exo oriR101 repA101(Ts); Ap^r	56
pAM238	IPTG-inducible expression vector; Spc^r; pSC101 derivative; low copy number	57
pREF71	pAM238::AUG-metK_Ec	This work
pBAD33	Arabinose-inducible expression vector; Cm^r; p15A derivative; low copy number	58
pREF73	pBAD33::GUG-metK_Ec	This work
pCtL2	10-fold coverage of C. trachomatis serovar L2 genome, 2.2-kb average insert size; Ap^r; pUC19 derivative, 500–700 copies per cell	3
pREF77	pUC19::ctl843_Ct; isolate 9	This work
pGEMT	PCR cloning vector; Ap^r; high copy number	Promega
pRAK367	pGEMT::lacI^q	This work
pBluescript II SK(+)	IPTG-inducible expression vector; Ap^r; pUC derivative; 300–500 copies per cell	Stratagene
pRAK368	pBluescript II SK(+)::samht_Ct lacI^q	This work