FIG 3 .
Western blot analysis of the whole-cell lysate (CL) of the overnight-grown wild-type M1-SF370 (M1-WT) and mutant (M1-SDHHBtail) GAS strains for the presence of SDH, CcpA, HPr, and CovR, using protein-specific antibodies. Equal amounts (50 µg total protein [25 ul] as the starting concentration) of cell lysates of M1-WT and M1-SDHHBtail were used for the assay. (A) Western blot analysis of equal volume (25ul) of the four serially 2-fold diluted samples of whole-cell lysates to determine the concentrations of different proteins as indicated in the wild-type and mutant GAS strains. (B) Reactivity of mono- and doubly phosphorylated forms of HPr (HPr-P1 and HPr-P2) in the whole-cell lysates of M1-WT and M1-SDHHBtail GAS strains.