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. 2011 May 31;2(3):e00068-11. doi: 10.1128/mBio.00068-11

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Copyright © 2011 Hong et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 4 — Effects of the cytoplasmic retention of SDH on the expression of virulence factors in GAS. (A) Transmission electron microscopy of the wild-type (M1-WT) and mutant (M1-SDH_HBtail) GAS strains. (B) Relative expression levels of the M1 protein and streptokinase in the cell wall fractions and culture supernatants of the M1-WT and M1-SDH_HBtail GAS strains as determined by type M1-reactive 10B6 monoclonal antibody (Anti-M 10B6 Ab) and antistreptokinase antibody (Anti-SKA Ab) in Western blot analysis. (C) Hyaluronic acid contents in M1-WT and M1-SDH_HBtail GAS strains. (D) Relative percent hemolysin activity on sheep RBCs of the serially diluted (10-fold-diluted) culture supernatants of M1-WT and M1-SDH_HBtail GAS strains. Lysis of RBCs in the undiluted culture supernatant of the M1-WT strain was set at 100%.