Skip to main content
. 2011 May 31;2(3):e00068-11. doi: 10.1128/mBio.00068-11

FIG 5 .

FIG 5

Relationship of the surface export of SDH and SpeB secretion. (A) Determination of the relative cysteine protease activities of SpeB present in the culture supernatants of M1-WT and M1-SDHHBtail GAS using FITC-labeled casein. The released fluorescence activity by the culture supernatant of the wild-type GAS strain was set at 100% activity, and its specificity was determined in the presence of cysteine protease inhibitor E-64. Values are means plus standard errors of the means (error bars) of three to six independent experiments. (B) Determination of SpeB-specific cysteine protease activity (hydrolysis of milk casein) present in the culture supernatants of M1-WT, M1-SDHHBtail, M1-SDHHBtail::sdh, and M1-WT::sdhHBtail GAS strains using milk agar. (C) Western blot analysis showing the effect on the secretion of SpeB in the culture supernatants of M1-WT, M1-SDHHBtail, M1-SDHHBtail::sdh, and M1-WT::sdhHBtail strains and the ability of SDH to bind secreted SpeB as determined by the blot overlay method.