FIG 4 .

Formation of high-molecular-mass complexes containing cross-linked rBclA and rBxpB. Complexes were formed in reaction mixtures containing 20 µM rBclA and 5 µM rBxpB. Samples of purified rBclA and rBxpB and of rBclA-rBxpB cross-linked complexes were separately analyzed in triplicate by SDS-PAGE. The three essentially identical gels were used to detect proteins and protein complexes by immunoblotting with either an anti-BclA or an anti-BxpB MAb or by staining with Coomassie blue.