TABLE 1 .
Bladder infection intensities and Tlr4 genotypes of female inbred mice and hybrids following intravesical inoculation with E. coli
Inbred mouse strain or hybrida |
No. of mice tested |
Tlr4
genotype |
CFU in bladderb |
---|---|---|---|
Inbred | |||
BALB/cAnN | 10 | Lpsn/Lpsn | 70 (1.84 ± 0.20) ABC |
C3H/HeJ | 6 | Lpsd/Lpsd | 533,921 (5.73 ± 0.60) AD |
C3H/HeOuJ | 6 | Lpsn/Lpsn | 489,778 (5.69 ± 0.20) BD |
Hybrid | |||
C3H/HeJ × BALB/cAnN | 15 | Lpsn/Lpsd | 44 (1.64 ± 0.09) EC |
C3H/HeJ × C3H/HeOuJ | 7 | Lpsn/Lpsd | 248,427 (5.40 ± 0.04) E |
Female mice from specific inbred strains (BALB/cAnN, C3H/HeJ, and C3H/HeOuJ) and hybrids bred from these strains [(C3H/HeJ × BALB/cAnN) and (C3H/HeJ × C3H/HeOuJ)].
Female mice were inoculated intravesically with 1 × 108 E. coli 1677 and euthanized 10 days later to assay for the total number of CFU in the bladder. Values are geometric means with mean log10 (CFU/mg of tissue) ± standard errors of the means in parentheses. Statistical analysis was conducted in two steps using SAS/STAT software, version 9.1 (SAS Institute, Inc.). The log-transformed CFU data were first analyzed by analysis of variance to estimate variability of values within and between experimental groups and then by Fisher’s protected least significant differences test to determine significant differences between groups. P values less than 0.05 were considered statistically significant. For comparisons between groups indicated by the letters A, B, and E, P is <0.001. For comparisons between groups indicated by the letters C and D, P is >0.05.