Figure 1.

The spatiotemporal regulation of the assembly and disassembly of endocytic factors. (A) Recruitment of endocytic machinery to the cortical endocytic sites. Note that a yeast dynamin-like protein Vps1 and the amphiphysins are recruited after initiation of actin assembly. (B) Uncoating of endocytic factors. The phosphorylation activity of the homologous protein kinases, Prk1 and Ark1, has been implicated in the disassembly of endocytic proteins, including Sla1, Pan1, Ent1 and Ent2. (C) The mean uncoating times for GFP-fused endocytic factors. The time spent by a post-internalized vesicle carrying GFP-fused protein in the cytoplasm was determined.