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Cortactin is co-phosphorylated on S405 and Y421. OSC19 oral squamous carcinoma cells serum starved for 24 h were stimulated with 100 ng/ml EGF for 20 min as indicated. Cell lysates were immunoprecipitated with anti-cortactin pS405 antibodies and analyzed by western blotting with anti-cortactin pY421 and pan-cortactin antibodies. Total cell lysates were evaluated for the presence of pS405 cortactin, pY421 cortactin, total cortactin and β-actin.
