Figure 7.

Using PhTx-433 to quantify GluA2-content in nucleated patches from wild-type and stargazer SCs. A, Representative example of a nucleated patch from a wild-type SC that was subjected to both a voltage ramp (top) and block by 100 nm PhTx-433 (bottom). In this case, the RI value was 0.34 and the current that remained unblocked by 100 nm PhTx-433 was 10.1%. B, In a representative example of a nucleated patch from a stargazer SC, the RI value was 0.17 and the remaining current was 12.2%. C, Bar graph comparing RI values from wild-type and stargazer SC nucleated patches, also subjected to block by 100 nm PhTx-433. Consistent with data from Figure 5, the average RI of wild-type (wt) SCs was significantly higher than that of stargazer (stg) SCs (wt: average RI = 0.40 ± 0.02, n = 19; stg: average RI = 0.27 ± 0.02, n = 15; p < 0.0001, Wilcoxon rank sum test). D, Bar graph showing that despite the significant difference in RI between wild-type and stargazer RIs, the current that remained unblocked in 100 nm PhTx-433 was not significantly different (wt: average remaining current = 21.0 ± 2.4%, n = 19; stg: average remaining current = 21.2 ± 3.3%, n = 15; N.S., Wilcoxon rank sum test). Open circles represent values from individual cells. Asterisks indicate significance, ***p < 0.0005, N.S. is not significant.