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. Author manuscript; available in PMC: 2011 May 31.
Published in final edited form as: Mol Microbiol. 2009 Dec 16;75(3):755–762. doi: 10.1111/j.1365-2958.2009.07016.x

Fig. 3. Electrophoretic mobility shift analysis (EMSA) of NrpR binding to operator DNA.

Fig. 3

A. DNA probes containing operators shown in Fig. 1: wild type (O1+O2), O2 only (O1 mutant), O1 only (O2 mutant), and both operators mutant. No (-) or 120 nM (+) NrpR was mixed with the DNA.

B. EMSA at different NrpR concentrations: O1+O2, 0, 0.05, 0.1, 0.15, 0.2, 0.4, 0.6, 1.0, and 1.5 nM; O2 and O1, 0, 0.25, 1, 2, 4, 8, 16, 32, and 64 nM.

C. Plot of data from B.