Skip to main content
. Author manuscript; available in PMC: 2011 May 31.
Published in final edited form as: Mol Microbiol. 2009 Dec 16;75(3):755–762. doi: 10.1111/j.1365-2958.2009.07016.x

Fig. 4. DNAse I footprinting analysis of NrpR binding to operator DNA.

Fig. 4

Footprints are delineated by bold lines and indicated with respect to the sequence. Double stranded DNA sequences on left and right represent O1+O2 and O1 only, respectively. Boxed sequences, TATA box; bent arrow, transcription start site; inverted arrow pairs, operator(s); asterisks, hypersensitive sites.

A, C and D. Lane 1, G+A ladder; lane 2, no NrpR; lane 3, 200 nM NrpR; lane 4, 400 nM NrpR. Units of DNAse I, 0.005

B. Lane 1, G+A ladder; lanes 2 and 3, no NrpR, lanes 4 and 5, 400 nM NrpR.; Units of DNAse I: lanes 2 and 4, 0.001; lanes 3 and 5, 0.002.